The membrane protein of SARS-CoV suppresses NF-κB activation
Identifieur interne : 003B15 ( Main/Exploration ); précédent : 003B14; suivant : 003B16The membrane protein of SARS-CoV suppresses NF-κB activation
Auteurs : XIAONAN FANG [République populaire de Chine] ; JINRONG GAO [République populaire de Chine] ; HONG ZHENG [République populaire de Chine] ; BAOZONG LI [République populaire de Chine] ; LINGBAO KONG [République populaire de Chine] ; YIJUAN ZHANG [République populaire de Chine] ; WEI WANG [République populaire de Chine] ; YINGCHUN ZENG [République populaire de Chine] ; LINBAI YE [République populaire de Chine]Source :
- Journal of medical virology [ 0146-6615 ] ; 2007.
Descripteurs français
- Pascal (Inist)
English descriptors
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Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) infects many organs, such as lung, liver, and immune organs and causes life-threatening atypical pneumonia, SARS causes high morbidity and mortality rates. The molecular mechanism of SARS pathogenesis remains elusive. Inflammatory stimuli can activate IKB kinase (IKK) signalsome and subsequently the nuclear factor kappa B (NF-κB), which influences gene expression of cyclooxygenase-2 (Cox-2) along with other transcription factors. In this work, we found that the membrane (M) protein of SARS-CoV physically interacted with IKKβ using a co-immunoprecipitation assay (IPA). Expression of M suppressed tumor necrosis factor alpha (TNF-α) induced NF-κB activation using a luciferase reporter assay. Further investigation showed M protein suppressed Cox-2 expression using a luciferase reporter gene assay, RT-PCR and Western blot analysis. The carboxyl terminal of M protein was sufficient for the M protein function. Together, these results indicate that SARS-CoV M suppresses NF-κB activity probably through a direct interaction with IKKβ, resulting in lower Cox-2 expression. Suppression of NF-KB activity and Cox-2 expression may contribute to SARS pathogenesis.
Affiliations:
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Life Science, Wuhan University</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Wei Wang" sort="Wei Wang" uniqKey="Wei Wang" last="Wei Wang">WEI WANG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, College of Life Science, Wuhan University</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region 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Wuhan</orgName></affiliation></author><author><name sortKey="Jinrong Gao" sort="Jinrong Gao" uniqKey="Jinrong Gao" last="Jinrong Gao">JINRONG GAO</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, College of Life Science, Wuhan University</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>College of Life Science, Wuhan University of Science and Technology Zhongnan Branch</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>2 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Hong Zheng" sort="Hong Zheng" uniqKey="Hong Zheng" last="Hong Zheng">HONG ZHENG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, College of Life Science, Wuhan University</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Baozong Li" sort="Baozong Li" uniqKey="Baozong Li" last="Baozong Li">BAOZONG LI</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, College of Life Science, Wuhan University</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Lingbao Kong" sort="Lingbao Kong" uniqKey="Lingbao Kong" last="Lingbao Kong">LINGBAO KONG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, College of Life Science, Wuhan University</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Yijuan Zhang" sort="Yijuan Zhang" uniqKey="Yijuan Zhang" last="Yijuan Zhang">YIJUAN ZHANG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, College of Life Science, Wuhan University</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Wei Wang" sort="Wei Wang" uniqKey="Wei Wang" last="Wei Wang">WEI WANG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, College of Life Science, Wuhan University</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Yingchun Zeng" sort="Yingchun Zeng" uniqKey="Yingchun Zeng" last="Yingchun Zeng">YINGCHUN ZENG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, College of Life Science, Wuhan University</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Linbai Ye" sort="Linbai Ye" uniqKey="Linbai Ye" last="Linbai Ye">LINBAI YE</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, College of Life Science, Wuhan University</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author></analytic><series><title level="j" type="main">Journal of medical virology</title><title level="j" type="abbreviated">J. med. virol.</title><idno type="ISSN">0146-6615</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of medical virology</title><title level="j" type="abbreviated">J. med. virol.</title><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Host virus relation</term><term>Membrane protein</term><term>Pathogenesis</term><term>Severe acute respiratory syndrome virus</term><term>Transcription factor NFκB</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term><term>Protéine membranaire</term><term>Facteur transcription NFκB</term><term>Relation hôte virus</term><term>Pathogénie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) infects many organs, such as lung, liver, and immune organs and causes life-threatening atypical pneumonia, SARS causes high morbidity and mortality rates. The molecular mechanism of SARS pathogenesis remains elusive. Inflammatory stimuli can activate IKB kinase (IKK) signalsome and subsequently the nuclear factor kappa B (NF-κB), which influences gene expression of cyclooxygenase-2 (Cox-2) along with other transcription factors. In this work, we found that the membrane (M) protein of SARS-CoV physically interacted with IKKβ using a co-immunoprecipitation assay (IPA). Expression of M suppressed tumor necrosis factor alpha (TNF-α) induced NF-κB activation using a luciferase reporter assay. Further investigation showed M protein suppressed Cox-2 expression using a luciferase reporter gene assay, RT-PCR and Western blot analysis. The carboxyl terminal of M protein was sufficient for the M protein function. Together, these results indicate that SARS-CoV M suppresses NF-κB activity probably through a direct interaction with IKKβ, resulting in lower Cox-2 expression. Suppression of NF-KB activity and Cox-2 expression may contribute to SARS pathogenesis.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Xiaonan Fang" sort="Xiaonan Fang" uniqKey="Xiaonan Fang" last="Xiaonan Fang">XIAONAN FANG</name></region><name sortKey="Baozong Li" sort="Baozong Li" uniqKey="Baozong Li" last="Baozong Li">BAOZONG LI</name><name sortKey="Hong Zheng" sort="Hong Zheng" uniqKey="Hong Zheng" last="Hong Zheng">HONG ZHENG</name><name sortKey="Jinrong Gao" sort="Jinrong Gao" uniqKey="Jinrong Gao" last="Jinrong Gao">JINRONG GAO</name><name sortKey="Jinrong Gao" sort="Jinrong Gao" uniqKey="Jinrong Gao" last="Jinrong Gao">JINRONG GAO</name><name sortKey="Linbai Ye" sort="Linbai Ye" uniqKey="Linbai Ye" last="Linbai Ye">LINBAI YE</name><name sortKey="Lingbao Kong" sort="Lingbao Kong" uniqKey="Lingbao Kong" last="Lingbao Kong">LINGBAO KONG</name><name sortKey="Wei Wang" sort="Wei Wang" uniqKey="Wei Wang" last="Wei Wang">WEI WANG</name><name sortKey="Yijuan Zhang" sort="Yijuan Zhang" uniqKey="Yijuan Zhang" last="Yijuan Zhang">YIJUAN ZHANG</name><name sortKey="Yingchun Zeng" sort="Yingchun Zeng" uniqKey="Yingchun Zeng" last="Yingchun Zeng">YINGCHUN ZENG</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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